Biochemical characterization of the POM-1 metallo-β-lactamase from Pseudomonas otitidis.
نویسندگان
چکیده
The POM-1 metallo-β-lactamase is a subclass B3 resident enzyme produced by Pseudomonas otitidis, a pathogen causing otic infections. The enzyme was overproduced in Escherichia coli BL21(DE3), purified by chromatography, and subjected to structural and functional analysis. The purified POM-1 is a tetrameric enzyme of broad substrate specificity with higher catalytic activities with penicillins and carbapenems than with cephalosporins.
منابع مشابه
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ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 59 3 شماره
صفحات -
تاریخ انتشار 2015